In this study, the effect of worksheets developed for chemical compounds included in General Chemistry class of Science education undergraduate programme in accordance with the constructivist learning theory on academic achievement and permanency was investigated. The subjects of the study were 80 first grade teacher candidates attending 19 Mayıs University, Education Faculty, Science and Technology Education Department. While the subject was taught to the students in the first group by the use of traditional instruction method, it was taught to the students in the second group by the use of worksheets. To obtain data, an achievement test consisting of 50 multiple-choice questions with a Cronbach-alpha value of 0.921 was applied as pre-test before the study, post-test at the end of the study and permanency test 5 weeks later after the study. Data were analyzed by the use of t-test in SPSS 15.0 package program. Study results revealed that experimental group students to whom subject was taught by the use of worksheets were more successful than the group students to whom subject was taught with traditional instruction method (t=23.230; p< .05) In the light of this data it was concluded that the use of worksheets as supplementary materials affect permanency positively (t=27.505; p < .05).