This study investigates undergraduate students’ perceptions of native speaker teachers (NST) and non-native speaker teachers (NNST) at three universities in Turkey. The study also aims to find out the effect of gender and the previous experience with non-native speaking teachers on the students’ perceptions of native and non-native instructors. 120 undergraduate university students, studying Japanese or English as a FL, were asked to rate a 30-item questionnaire related to, in-class teaching roles, in-class management roles, in-class communication skills, and individual qualities of native and non-native speaking teachers’. Results indicated that, students perceive NSTs as superior to NNSTs only in terms of in-class teaching roles. In addition to that, male students possessed positive attitudes both towards NSTs and NNSTs. And finally the study found that number of NSTs that the students previously studied with is an important factor that influences students’ opinions.